Reverse Transcription with MMLV Reverse Transcriptase īiotin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) Primer Extension with Klenow 3'-5' exo. Nick Translation with DNAse I/ DNA Polymerase I & in-house data PCR with Taq polymerase & in-house data Molecular Weight: 862.67 g/mol (free acid)įorm: filtered solution (30 kDa) in 10 mM Tris-HCl Molecular Formula: C 28H 45N 6O 17P 3S (free acid) Shelf Life: 12 months after date of delivery Short term exposure (up to 1 week cumulative) to ambient temperature possible.
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